Molecular Biology Techniques
This biology techniques introductory laboratory course is designed to provide experience of analytical and preparative techniques for proteins and nucleic acids Accredited for CME by the Royal College of Pathologists and for CPD by the Institute of Biomedical Science.
Nucleic acid techniques
- Chip technology and DNA arrays including quantification of RNA and DNA using microfluidics
- Quantitative RT PCR
- Introduction to Bioinformatics and Genomics
- Reverse transcriptase PCR (RT-PCR)
- ECL detection of nucleic acids
- RNA isolation, purification and analysis
- Nucleic acid blotting and hybridization
- Preparation of labelled probes
- Bacterial transformation and electroporation
- Automated DNA sequencing
- PCR and primer design
- Cloning and cDNA library screening
- Plasmid DNA isolation (Qiagen column) and analysis
Protein techniques
- Mamalian transfection
- Expression/reporter gene vectors
- Real time PCR and Taqman
- 2-D PAGE and Proteomics
- Functional Proteomics
- Mass Spectrometry and Proteomics
- SDS-PAGE (linear and gradient)
- Protein bioinformatics
- Protein (western) blotting with immuno- detection and enhanced chemiluminescence
- Silver staining of protein gels
Why choose this course?
learn: An introductory, predominantly laboratory-based course providing experience of analytical and preparative techniques for proteins and nucleic acids.
Course for: The course is suitable for those not necessarily engaged in full-time laboratory work, but also of interest to research workers especially in the medical/clinical area.
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Course fees
£750 (excluding VAT)
Fee includes light refreshments, buffet-style lunch each day, optional evening course dinner on the first night and a comprehensive course manual.
Study routes
- Part Time